PARD3 Antibody
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB |
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Primary Accession | Q99NH2 |
Other Accession | A5D6P2 |
Reactivity | Human, Mouse, Rat |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 149075 Da |
Gene ID | 93742 |
---|---|
Positive Control | 3T3 cell lysate |
Application & Usage | The antibody can be used for Western blot analysis (1-4 µg/ml). However, the optimal conditions should be determined individually. Blocking peptide is available separately. |
Other Names | Par-3 (Partitioning defective 3) homolog |
Target/Specificity | PARD3 |
Antibody Form | Liquid |
Appearance | Colorless liquid |
Formulation | 100 µg (0.5 mg/ml) affinity purified rabbit anti-PARD3 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 5 mM EDTA and 0.01% thimerosal. |
Handling | The antibody solution should be gently mixed before use. |
Reconstitution & Storage | -20 °C |
Background Descriptions | |
Precautions | PARD3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | Pard3 |
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Synonyms | Par3 |
Function | Adapter protein involved in asymmetrical cell division and cell polarization processes (By similarity). Seems to play a central role in the formation of epithelial tight junctions (By similarity). Targets the phosphatase PTEN to cell junctions (By similarity). Association with PARD6B may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly (PubMed:11839275). The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins (By similarity). Required for establishment of neuronal polarity and normal axon formation in cultured hippocampal neurons (By similarity). Involved in Schwann cell peripheral myelination (PubMed:21949390). |
Cellular Location | Cytoplasm. Endomembrane system. Cell junction. Cell junction, tight junction. Cell junction, adherens junction. Cytoplasm, cell cortex. Cytoplasm, cytoskeleton. Cell membrane. Note=Localized along the cell-cell contact region. Colocalizes with PARD6A and PRKCI at epithelial tight junctions. Colocalizes with the cortical actin that overlays the meiotic spindle during metaphase I and metaphase II Presence of KRIT1, CDH5 and RAP1B is required for its localization to the cell junction (By similarity). Colocalized with SIRT2 in internode region of myelin sheath. |
Tissue Location | All isoforms are expressed in heart, while expression in brain is mainly limited to isoform 1, and to isoform 3 to a weaker level |
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Provided below are standard protocols that you may find useful for product applications.
Background
PARD (partitioning-defective) proteins are important in asymmetric cell division and polarized growth. PARD3 plays a vital role in the formation of epithelial tight junctions. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins.
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