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HADH/HCDH Antibody

Rabbit Polyclonal Antibody

     
  • WB - HADH/HCDH Antibody ABV10208-30
    Western blot analysis of HADH/HCDH using Jurkat cell lysate (Lane 1), mouse 3T3 cell lysate (Lane 2) and rat kidney tissue lysate (Lane 3).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession Q9WVK7
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 34448 Da
Additional Information
Gene ID 113965
Positive Control Jurkat cell lysate, mouse 3T3 cell lysate, and rat kidney tissue lysate
Application & Usage The antibody can be used for Western blot analysis (1-4 µg/ml). However, the optimal conditions should be determined individually. Blocking peptide is available separately.
Other Names Hydroxyacyl-coenzyme A dehydrogenase
Target/Specificity HADH/HCDH
Antibody Form Liquid
Appearance Colorless liquid
Formulation 100 µg (0.5 mg/ml) affinity purified rabbit anti-HADH/HCDH polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 5 mM EDTA and 0.01% thimerosal.
Handling The antibody solution should be gently mixed before use.
Reconstitution & Storage -20 °C
Background Descriptions
PrecautionsHADH/HCDH Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Hadh
Synonyms Had, Hadhsc, Schad
Function Mitochondrial fatty acid beta-oxidation enzyme that catalyzes the third step of the beta-oxidation cycle for medium and short-chain 3-hydroxy fatty acyl-CoAs (C4 to C10) (By similarity). Plays a role in the control of insulin secretion by inhibiting the activation of glutamate dehydrogenase 1 (GLUD1), an enzyme that has an important role in regulating amino acid-induced insulin secretion (By similarity).
Cellular Location Mitochondrion matrix {ECO:0000250|UniProtKB:P00348}
Citations (0)
citation

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Background

L-3-hydroxyacyl-CoA dehydrogenase (HADH/HCDH) is a mitochondrial matrix protein expressed in pancreas, liver, heart, kidney and skeletal muscle. L-3-hydroxyacyl-CoA dehydrogenase catalyzes the oxidation of straight-chain 3-hydroxyacyl-CoAs as part of the beta-oxidation pathway. Its enzymatic activity is highest with medium-chain-length fatty acids. Mutations in this gene can lead to HADH (3-α-hydroxyacyl-CoA dehydrogenase) deficiency and familial hyperinsulinemic hypoglycemia 4 (HHF4).

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Discontinued
Cat# ABV10208-30
Size:

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