|Application ||WB, IP|
|Calculated MW||132336 Da|
|Application & Usage||Western blot analysis (1 µg/ml) and immunoprecipitation. However, the optimal conditions should be determined individually.|
|Other Names||RGS3, 602189, P49796, C2PA, FLJ20370, FLJ31516, FLJ90496, PDZ-RGS3, RGP3|
|Formulation||100 µg (0.2 mg/ml) protein A-purified rabbit anti-RGS3 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% sodium azide.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||RGS3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Down-regulates signaling from heterotrimeric G-proteins by increasing the GTPase activity of the alpha subunits, thereby driving them into their inactive GDP-bound form. Down-regulates G- protein-mediated release of inositol phosphates and activation of MAP kinases.|
|Cellular Location||Cytoplasm. Nucleus. Cell membrane; Peripheral membrane protein. Note=Long isoforms are cytoplasmic and associated with the plasma membrane (PubMed:9858594). Short isoforms are nuclear (PubMed:10749886)|
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Provided below are standard protocols that you may find useful for product applications.
RGS3 (regulator of G protein signaling 3) is a 519-residue protein that is widely expressed in many tissues and cells. The protein contains an RGS domain near the carboxy terminus that serves as a GTPase-activating protein (GAP). RGS3 negatively regulates the activation of Giα and Gαq. A truncated RGS3 (RGS3T), with its N-terminal 313 residues removed, has been shown to negatively regulate acenylyl cyclase and PLC. RGS3T may be localized in the nucleus and RGS3 is found in cytoplasm. Translocation to membrane is important for the RGS proteins to interact with heterotrimeric G proteins.
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