|Application ||WB, IHC|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||26820 Da|
|Application & Usage||Western blotting (0.5-4 µg/ml) and Immunohistochemistry (5-20 µg/ml). However, the optimal conditions should be determined individually. The immunoaffinity purified antibody detects the 25 kDa Smac/DIABLO in samples from human, mouse, and rat origins.|
|Other Names||SMAC, SMAC3, DIABLO, DIABLO-S , FLJ10537, FLJ25049, 0610041G12Rik|
|Formulation||100 µg (0.5 mg/ml) peptide affinity purified rabbit anti-Smac/DIABLO polyclonal antibody in PBS (pH 7.2) containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||Smac/DIABLO Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP). Inhibits the activity of BIRC6/bruce by inhibiting its binding to caspases (By similarity).|
|Cellular Location||Mitochondrion. Note=Released into the cytosol when cells undergo apoptosis|
|Tissue Location||Highest expression found in heart, liver, kidney and testis.|
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Provided below are standard protocols that you may find useful for product applications.
Smac/DIABLO is a mitochondrial protein that is released along with cytochrome c during apoptosis and activates cytochrome c/Apaf-1/caspase-9 pathway. Smac/DIABLO binds to IAPs (Inhibitor of Apoptosis Proteins) and neutralizes the inhibitory effect of IAPs. Analysis of the structural basis of Smac/DIABLO has revealed that the N-terminal amino acids are required for Smac/DIABLO to bind to IAPs and activation of caspases.
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