|Reactivity||Human, Mouse, Rat|
|Calculated MW||78436 Da|
|Positive Control||3T3 cell lysate|
|Application & Usage||Western blot: 1:200|
|Other Names||DNA cross-link repair 1C protein, SNM1-like protein|
|Formulation||100 µg (0.5 mg/ml) of antibody in PBS, 0.01 % BSA, 0.01 % thimerosal, and 50 % glycerol, pH 7.2|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||Artemis (DCLREIC) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||ARTEMIS, ASCID, SCIDA, SNM1C|
|Function||Required for V(D)J recombination, the process by which exons encoding the antigen-binding domains of immunoglobulins and T-cell receptor proteins are assembled from individual V, (D), and J gene segments. V(D)J recombination is initiated by the lymphoid specific RAG endonuclease complex, which generates site specific DNA double strand breaks (DSBs). These DSBs present two types of DNA end structures: hairpin sealed coding ends and phosphorylated blunt signal ends. These ends are independently repaired by the non homologous end joining (NHEJ) pathway to form coding and signal joints respectively. This protein exhibits single-strand specific 5'-3' exonuclease activity in isolation and acquires endonucleolytic activity on 5' and 3' hairpins and overhangs when in a complex with PRKDC. The latter activity is required specifically for the resolution of closed hairpins prior to the formation of the coding joint. May also be required for the repair of complex DSBs induced by ionizing radiation, which require substantial end-processing prior to religation by NHEJ.|
|Tissue Location||Ubiquitously expressed, with highest levels in the kidney, lung, pancreas and placenta (at the mRNA level) Expression is not increased in thymus or bone marrow, sites of V(D)J recombination.|
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Artemis is a 78 kDa protein, belonging to metallo-beta-lactamase superfamily. It is involved in endonuclease activity to break DNA hairpins. It also has been reported that the C-terminus of Artemis interacts with DNA binding domain of Ligase IV directly. Ligase IV is a key enzyme in DNA repair and V(D)J recombination. Thus, Artemis is a novel protein that is involved in V(D)J recombination/DNA repair. Mutations cause human T-B-severe combined immunodeficiency associated with increased cellular radiosensitivity (RS-SCID).
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