|Calculated MW||43262 Da|
|Application & Usage||Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. 293 cell lysate can be used as a positive control. Detects the 43 kDa human caspase-4|
|Other Names||CASP-4, ICH-2 protease, TX protease, ICH2|
|Formulation||100 µg (0.5 mg/ml) immunoaffinity purified mouse IgG in PBS containing 0.5% BSA, 1.5 mM sodium azide and 30% glycerol.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||Caspase-4 Antibody (Clone D44) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Involved in the activation cascade of caspases responsible for apoptosis execution. Involved in ER-stress induced apoptosis. Cleaves caspase-1.|
|Cellular Location||Endoplasmic reticulum membrane. Mitochondrion|
|Tissue Location||Widely expressed, with highest levels in spleen and lung. Moderate expression in heart and liver, low expression in skeletal muscle, kidney and testis. Not found in the brain|
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Provided below are standard protocols that you may find useful for product applications.
The caspase family of cysteine proteases play a key role in apoptosis. Like other caspases in the family, caspase-4 is also synthesized as inactive pro-enzyme that is processed in cells by self-proteolysis and/or cleavage by another upstream protease. The processed form of caspase-4 consists of large and small subunits which associate to form an active enzyme. Caspase-4 has been implicated involving in Fas-mediated apoptosis.
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