|Application ||WB, IHC, IP|
|Calculated MW||31485 Da|
|Application & Usage||Western blotting (1-4 µg/ml), immunoprecipitation, and Immunohistochemistry. However, the optimal conditions should be determined individually. HeLa cell lysate can be used as a positive control and an approximately 40 kDa band should be detected by Western blotting.|
|Other Names||FASLG, FASL , FasL, CD95L , APT1LG1, CD178 , APTL , TNFSF6|
|Formulation||100 µg (0.5 mg/ml) Protein G purified mouse monoclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||Fas Ligand Antibody (Clone I-6060) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||APT1LG1, CD95L, FASL, TNFSF6|
|Function||Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells. May be involved in cytotoxic T-cell mediated apoptosis and in T-cell development. TNFRSF6/FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. Binding to the decoy receptor TNFRSF6B/DcR3 modulates its effects.|
|Cellular Location||Cell membrane; Single-pass type II membrane protein. Cytoplasmic vesicle lumen. Lysosome lumen. Note=Is internalized into multivesicular bodies of secretory lysosomes after phosphorylation by FGR and monoubiquitination. Colocalizes with the SPPL2A protease at the cell membrane FasL intracellular domain: Nucleus. Note=The FasL ICD cytoplasmic form is translocated into the nucleus|
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Provided below are standard protocols that you may find useful for product applications.
Fas (APO-1, CD95) is a 45 kDa cell surface protein that mediates apoptosis when cross-linked with agonistic anti-Fas antibodies or Fas ligand (FasL). FasL is a tumor necrosis factor (TNF)-related type II membrane protein and is expressed in various tissue and cells. Both Fas and FasL are tho µght to play an important role in the apoptosis processes that takes place during T cell development.
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