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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   BMF   


Rabbit Polyclonal Antibody

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q96LC9
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 20508 Da
Additional Information
Gene ID 90427
Positive Control Western Blot: Jurkat cell lysate and Rat kidney tissue lysate. IHC: Kidney tissue
Application & Usage Western blotting (0.5-4 µg/ml) and Immunohistochemistry (5 µg/ml). However, the optimal conditions should be determined individually. The antibody recognizes ~28 kDa BMF in sample from human, mouse and rat origins. Reactivity to other species has not been tested.
Other Names BMF, FLJ00065, Bcl-2-modifying factor
Target/Specificity BMF
Antibody Form Liquid
Appearance Colorless liquid
Formulation 100 µg (0.5 mg/ml) affinity purified rabbit anti-BMF polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.
Handling The antibody solution should be gently mixed before use.
Reconstitution & Storage -20 °C
Background Descriptions
PrecautionsBMF is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name BMF
Function May play a role in apoptosis. Isoform 1 seems to be the main initiator.
Tissue Location Isoform 1 is mainly expressed in B-lymphoid cells. Isoform 2 and isoform 3 are mainly expressed in B-CLL and normal B-cells.
Research Areas
Citations (0)

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Bmf (for Bcl-2-modifying factor) is a novel BH3-only protein recently identified in human and mouse. The BH3 domain in Bmf is required for both binding to Bcl-2 proteins and for triggering apoptosis. In healthy cells, Bmf associates with the dynein light chain 2 (DLC2) component of the myosin V motors and is sequestered by the cell's actin cytoskeleton. Disruption of the actin cytoskeleton, either by depolymerization of actin filaments or by detachment of cells from the extracellular matrix, triggers release and activation of Bmf, initiating the downstream apoptosis program.

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$ 245.00
Cat# ABV10244
(40 western blots)
Availability: 5-7days
Bulk Size
Seasonal Special on Bulk Order
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