|Application ||WB, IHC|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||20508 Da|
|Positive Control||Western Blot: Jurkat cell lysate and Rat kidney tissue lysate. IHC: Kidney tissue|
|Application & Usage||Western blotting (0.5-4 µg/ml) and Immunohistochemistry (5 µg/ml). However, the optimal conditions should be determined individually. The antibody recognizes ~28 kDa BMF in sample from human, mouse and rat origins. Reactivity to other species has not been tested.|
|Other Names||BMF, FLJ00065, Bcl-2-modifying factor|
|Formulation||100 µg (0.5 mg/ml) affinity purified rabbit anti-BMF polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||BMF is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||May play a role in apoptosis. Isoform 1 seems to be the main initiator.|
|Tissue Location||Isoform 1 is mainly expressed in B-lymphoid cells. Isoform 2 and isoform 3 are mainly expressed in B-CLL and normal B-cells.|
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Provided below are standard protocols that you may find useful for product applications.
Bmf (for Bcl-2-modifying factor) is a novel BH3-only protein recently identified in human and mouse. The BH3 domain in Bmf is required for both binding to Bcl-2 proteins and for triggering apoptosis. In healthy cells, Bmf associates with the dynein light chain 2 (DLC2) component of the myosin V motors and is sequestered by the cell's actin cytoskeleton. Disruption of the actin cytoskeleton, either by depolymerization of actin filaments or by detachment of cells from the extracellular matrix, triggers release and activation of Bmf, initiating the downstream apoptosis program.
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