|Reactivity||Human, Mouse, Rat|
|Application & Usage||Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The antibody recognizes ~35 kDa EndoG of human, mouse, and rat origins. Reactivity to other species has not been tested.|
|Other Names||ENDOG , FLJ27463,|
|Formulation||100 µg (0.5 mg/ml) affinity purified rabbit anti-EndoG polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||EndoG Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
Endonuclease G (EndoG) is a mitochondrion-specific nuclease that specifically activated by apoptotic stimuli and translocates to the nucleus during apoptosis. In nucleus, EndoG induces nucleosomal fragmentation of DNA independent of caspase and DFF/CAD.
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