|Application ||WB, IHC|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||27366 Da|
|Application & Usage||Western blotting (0.5-4 µg/ml) and Immunohistochemistry (5-10 µg/ml). However, the optimal dilution conditions should be determined individually. Human Lung tissue lysate can be used as a positive control. The antibody detects the 27 kDa p53DINP1-α in human, mouse, and rat samples. A lower band (~18 kDa) which represents the p53DINP1-β was also detected in certain tissue and cells.|
|Other Names||TP53DINP1 Teap, TP53DINP1A, p53DINP1, P53DINP1, TP53DINP1, SIP , FLJ22139, DKFZp434M1317, TP53INP1B|
|Formulation||100 µg(0.5 mg/ml) affinity purified rabbit anti-p53DINP1 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||p53DINP1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Antiproliferative and proapoptotic protein involved in cell stress response which acts as a dual regulator of transcription and autophagy. Acts as a positive regulator of autophagy. In response to cellular stress or activation of autophagy, relocates to autophagosomes where it interacts with autophagosome-associated proteins GABARAP, GABARAPL1/L2, MAP1LC3A/B/C and regulates autophagy. Acts as an antioxidant and plays a major role in p53/TP53-driven oxidative stress response. Possesses both a p53/TP53-independent intracellular reactive oxygen species (ROS) regulatory function and a p53/TP53-dependent transcription regulatory function. Positively regulates p53/TP53 and p73/TP73 and stimulates their capacity to induce apoptosis and regulate cell cycle. In response to double-strand DNA breaks, promotes p53/TP53 phosphorylation on 'Ser-46' and subsequent apoptosis. Acts as a tumor suppressor by inducing cell death by an autophagy and caspase-dependent mechanism. Can reduce cell migration by regulating the expression of SPARC.|
|Cellular Location||Cytoplasm, cytosol. Nucleus. Nucleus, PML body. Cytoplasmic vesicle, autophagosome. Note=Shuttles between the nucleus and the cytoplasm, depending on cellular stress conditions, and re-localizes to autophagosomes on autophagy activation|
|Tissue Location||Ubiquitously expressed.|
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Provided below are standard protocols that you may find useful for product applications.
p53DINP1 (p53-dependent damage-inducible nuclear protein 1) encodes two proteins termed p53DINP1-α (27 kDa) and p53DINP1-β (18 kDa). p53DINP1 may regulate p53-dependent apoptosis thro µgh phosphorylation at Ser46 and induction of p53AIP1. p53DINP1 is expressed in many tissues and induced by a variety of stress agents including UV stress, mutagenic stress, heat shock, and oxidative stress.
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