|Application ||WB, IHC|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||76472 Da|
|Application & Usage||Western blotting (0.5-4 µg/ml). Based on researchers feed back, it also works in Immunohistochemistry (10-20 µg/ml). However, the optimal conditions should be determined individually.|
|Other Names||POU2F1, OCT1, OTF1, OTF-1, NF-A1 , Oct-1, 5451|
|Formulation||100 µg (0.5 mg/ml) affinity purified rabbit anti-Oct-1 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||Oct-1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and of genes such as those for histone H2B and immunoglobulins. Modulates transcription transactivation by NR3C1, AR and PGR (By similarity). In case of human herpes simplex virus (HSV) infection, POU2F1 forms a multiprotein-DNA complex with the viral transactivator protein VP16 and HCFC1 thereby enabling the transcription of the viral immediate early genes.|
|Tissue Location||Ubiquitous. Isoform 2 is lymphocyte-specific.|
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Provided below are standard protocols that you may find useful for product applications.
Members of Oct family of transcription factors specifically interact with Octamer motif ATGCAAAT, a regulatory element important for tissue- and cell-specific transcription as well as for transcription of a number of housekeeping genes. All of the members of the Oct family contain two highly conserved domains which are separated by 14-26 variable amino acids. These include the POU homeodomain and the POU-specific domain. Both are required for DNA binding and are involved in protein-protein interactions. Evidences indicate that regulation of Oct family transcription factors occurs at the level of phosphorylation.
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