|Calculated MW||31442 Da|
|Application & Usage||Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. Detects ~31 kDa FasL in samples of mouse and rat origins. A ~70 kDa band, possible represents the FasL and Fas complex can also be detected|
|Other Names||FASLG, FASL , FasL, CD95L , APT1LG1, CD178 , APTL , TNFSF6|
|Formulation||100 µg (0.5 mg/ml) affinity purified rabbit anti-FasL polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||FasL Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||Apt1lg1, Cd95l, Fasl, gld, Tnfsf6|
|Function||Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells. May be involved in cytotoxic T-cell mediated apoptosis and in T-cell development. TNFRSF6/FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. Binding to the decoy receptor TNFRSF6B/DcR3 modulates its effects (By similarity).|
|Cellular Location||Isoform FasL: Cell membrane; Single-pass type II membrane protein. Cytoplasmic vesicle lumen Lysosome lumen. Note=Is internalized into multivesicular bodies of secretory lysosomes after phosphorylation by FGR and monoubiquitination. Colocalizes with the SPPL2A protease at the cell membrane (By similarity). Tumor necrosis factor ligand superfamily member 6, soluble form: Secreted. Note=May be released into the extracellular fluid, probably by cleavage form the cell surface|
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Provided below are standard protocols that you may find useful for product applications.
Fas/Apo-1/CD95 is a cell surface type-I membrane glycoprotein with an apparent molecular weight of 44 kDa on SDS-PAGE. Fas is a member of the TNFR family that includes TNFR1, TNFR2, CD27, CD30, and CD40. Binding of Fas-ligand (FasL) to Fas or crosslinking of Fas by anti-Fas monoclonal antibodies leads to apoptosis in Fas expressing cells. Both Fas and TNF-R contain the 70 amino acids cytoplasmic “death domain” that is responsible for transmitting signal for apoptosis.
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