FasL Antibody
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB |
---|---|
Primary Accession | P41047 |
Reactivity | Mouse, Rat |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 31442 Da |
Gene ID | 14103 |
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Application & Usage | Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. Detects ~31 kDa FasL in samples of mouse and rat origins. A ~70 kDa band, possible represents the FasL and Fas complex can also be detected |
Other Names | FASLG, FASL , FasL, CD95L , APT1LG1, CD178 , APTL , TNFSF6 |
Target/Specificity | FasL |
Antibody Form | Liquid |
Appearance | Colorless liquid |
Formulation | 100 µg (0.5 mg/ml) affinity purified rabbit anti-FasL polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal. |
Handling | The antibody solution should be gently mixed before use. |
Reconstitution & Storage | -20 °C |
Background Descriptions | |
Precautions | FasL Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | Faslg |
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Synonyms | Apt1lg1, Cd95l, Fasl, gld, Tnfsf6 |
Function | Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells (PubMed:7511063). Involved in cytotoxic T-cell-mediated apoptosis, natural killer cell-mediated apoptosis and in T-cell development (PubMed:19794494, PubMed:7532682). Initiates fratricidal/suicidal activation-induced cell death (AICD) in antigen-activated T-cells contributing to the termination of immune responses (PubMed:19794494). TNFRSF6/FAS-mediated apoptosis has also a role in the induction of peripheral tolerance (PubMed:10779162). Binds to TNFRSF6B/DcR3, a decoy receptor that blocks apoptosis (By similarity). |
Cellular Location | [Isoform FasL]: Cell membrane; Single-pass type II membrane protein. Cytoplasmic vesicle lumen {ECO:0000250|UniProtKB:P48023}. Lysosome lumen {ECO:0000250|UniProtKB:P48023}. Note=Is internalized into multivesicular bodies of secretory lysosomes after phosphorylation by FGR and monoubiquitination. Colocalizes with the SPPL2A protease at the cell membrane. {ECO:0000250|UniProtKB:P48023} [FasL intracellular domain]: Nucleus {ECO:0000250|UniProtKB:P48023}. Note=The FasL ICD cytoplasmic form is translocated into the nucleus. {ECO:0000250|UniProtKB:P48023} |
Tissue Location | Expressed in T-cells (PubMed:19794494). Expressed in natural killer cells (PubMed:7532682). |
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Provided below are standard protocols that you may find useful for product applications.
Background
Fas/Apo-1/CD95 is a cell surface type-I membrane glycoprotein with an apparent molecular weight of 44 kDa on SDS-PAGE. Fas is a member of the TNFR family that includes TNFR1, TNFR2, CD27, CD30, and CD40. Binding of Fas-ligand (FasL) to Fas or crosslinking of Fas by anti-Fas monoclonal antibodies leads to apoptosis in Fas expressing cells. Both Fas and TNF-R contain the 70 amino acids cytoplasmic “death domain” that is responsible for transmitting signal for apoptosis.
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