|Application ||WB, IHC, IP|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||47816 Da|
|Application & Usage||Western blotting (0.5-4 µg/ml) and in immunoprecipitation (10-20 µg/ml), and Immunohistochemistry (10-20 µg/ml). However, the optimal conditions should be determined individually. Mouse small intestine tissue lysate can be used as a positive control.|
|Other Names||TANK (NT), TRAF family member-associated NF-kappaB activator, I-TRAF|
|Formulation||100 µg (0.2 mg/ml) affinity purified rabbit anti-TANK polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||TANK Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Adapter protein involved in I-kappa-B-kinase (IKK) regulation which constitutively binds TBK1 and IKBKE playing a role in antiviral innate immunity. Acts as a regulator of TRAF function by maintaining them in a latent state. Blocks TRAF2 binding to LMP1 and inhibits LMP1-mediated NF-kappa-B activation. Negatively regulates NF-kappaB signaling and cell survival upon DNA damage (PubMed:25861989). Plays a role as an adapter to assemble ZC3H12A, USP10 in a deubiquitination complex which plays a negative feedback response to attenuate NF-kappaB activation through the deubiquitination of IKBKG or TRAF6 in response to interleukin-1-beta (IL1B) stimulation or upon DNA damage (PubMed:25861989). Promotes UBP10-induced deubiquitination of TRAF6 in response to DNA damage (PubMed:25861989). May control negatively TRAF2-mediated NF-kappa-B activation signaled by CD40, TNFR1 and TNFR2.|
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Provided below are standard protocols that you may find useful for product applications.
Members of TRAF family associate with activated TNF-R and CD40 and have been implicated to induce nuclear translocation of NFkB. TANK can bind to all three TRAFs and synergizing with TRAF2 to activate the NFkB signal cascade.
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