|Application ||WB, IHC, IP|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||95348 Da|
|Application & Usage||Western blotting (0.5-4 µg/ml), immunoprecipitation (5-10 µg/ml), and Immunohistochemistry (10-20 µg/ml). However, the optimal conditions should be determined individually.|
|Other Names||Cell adhesion molecules, BL-CAM , SIGLEC-2 , Siglec-2, SIGLEC2, CD22, FLJ22814 , MGC130020, Leu-14,|
|Formulation||100 µg (0.2 mg/ml) affinity purified rabbit anti-BLCAM polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||BLCAM Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules.|
|Cellular Location||Cell membrane; Single-pass type I membrane protein|
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Provided below are standard protocols that you may find useful for product applications.
Cell adhesion molecules (CAMs) are a family of closely related cell surface glycoproteins involved in cell-cell interactions during growth and are tho µght to play an important role in embryogenesis and development. BLCAM α and BLCAM β are produced by alternative splicing of the CD22 gene and form a heterodimer that mediates B cell-B cell interactions.
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