|Reactivity||Human, Mouse, Rat|
|Calculated MW||79995 Da|
|Application & Usage||Western blotting (1:200-1000 dilutions). However, the optimal concentrations should be determined individually. The anti-calpain 2 antibody recognizes the 80 kDa Calpain 2. It does not cross-react with other calpains|
|Other Names||M-Calpain , Calpain 2 , Calpian II , CANPL2 , CANP2 , millimolar-calpain , mCANP , FLJ39928 , CANPml , EC 220.127.116.11|
|Formulation||100 µl affinity purified rabbit polyclonal anti-calpain 2 antibody in phosphate-buffered saline (PBS) containing 50% glycerol, 1% BSA, and 0.02% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||Calpain-2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves MYOC at 'Arg-226' (PubMed:17650508). Proteolytically cleaves CPEB3 following neuronal stimulation which abolishes CPEB3 translational repressor activity, leading to translation of CPEB3 target mRNAs (By similarity).|
|Cellular Location||Cytoplasm. Cell membrane. Note=Translocates to the plasma membrane upon Ca(2+) binding|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
firstname.lastname@example.org, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Calpains are cytosolic, calcium-dependent, cysteine proteases that are ubiquitously expressed in mammalian and avian tissues. Calpain-1 and 2 consist of a common small subunit and a large variable subunit. Domains in the large subunit include the amino terminal domain I, the proteinase domain II, domain III, and the EF-hand domain IV. Type I calpain requires micromolar amounts of calcium, while type II calpain requires millimolar amounts of calcium.
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at email@example.com.