Calpain-2 Antibody
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB |
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Primary Accession | P17655 |
Other Accession | NP_001739 |
Reactivity | Human, Mouse, Rat |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 79995 Da |
Gene ID | 824 |
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Application & Usage | Western blotting (1:200-1000 dilutions). However, the optimal concentrations should be determined individually. The anti-calpain 2 antibody recognizes the 80 kDa Calpain 2. It does not cross-react with other calpains |
Other Names | M-Calpain , Calpain 2 , Calpian II , CANPL2 , CANP2 , millimolar-calpain , mCANP , FLJ39928 , CANPml , EC 3.4.22.53 |
Target/Specificity | Calpain 2 |
Antibody Form | Liquid |
Appearance | Colorless liquid |
Formulation | 100 µl affinity purified rabbit polyclonal anti-calpain 2 antibody in phosphate-buffered saline (PBS) containing 50% glycerol, 1% BSA, and 0.02% thimerosal. |
Handling | The antibody solution should be gently mixed before use. |
Reconstitution & Storage | -20 °C |
Background Descriptions | |
Precautions | Calpain-2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | CAPN2 |
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Synonyms | CANPL2 |
Function | Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves MYOC at 'Arg-226' (PubMed:17650508). Proteolytically cleaves CPEB3 following neuronal stimulation which abolishes CPEB3 translational repressor activity, leading to translation of CPEB3 target mRNAs (By similarity). |
Cellular Location | Cytoplasm. Cell membrane. Note=Translocates to the plasma membrane upon Ca(2+) binding |
Tissue Location | Ubiquitous. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Calpains are cytosolic, calcium-dependent, cysteine proteases that are ubiquitously expressed in mammalian and avian tissues. Calpain-1 and 2 consist of a common small subunit and a large variable subunit. Domains in the large subunit include the amino terminal domain I, the proteinase domain II, domain III, and the EF-hand domain IV. Type I calpain requires micromolar amounts of calcium, while type II calpain requires millimolar amounts of calcium.
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