Phospho-CaMKII Antibody
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB |
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Primary Accession | Q00168.1 |
Other Accession | ACZ95102.1 |
Reactivity | Human, Mouse, Rat |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Application & Usage | Western blotting at 1-2 µg/ml. However, the optimal concentrations should be determined individually. The anti-Phospho-CaMKII antibody recognizes only the phosphorylated CaMKII (Thr286) in samples from human, mouse, and rat origins. |
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Other Names | CAMK2A , CAMKA , KIAA0968, EC 2.7.11.17 |
Target/Specificity | CaMKII |
Antibody Form | Liquid |
Appearance | Colorless liquid |
Formulation | 100 µl purified rabbit polyclonal anti-Phospho-CaMKII (Thr286) antibody in phosphate-buffered saline (PBS) containing 50% glycerol, 1% BSA, and 0.02% thimerosal. |
Handling | The antibody solution should be gently mixed before use. |
Reconstitution & Storage | -20 °C |
Background Descriptions | |
Precautions | Phospho-CaMKII Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
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Provided below are standard protocols that you may find useful for product applications.
Background
CaMKII is a member of calcium/calmodulin-activated kinase, functioning in neural synaptic stimulation and T-cell receptor signaling. CaMKII has catalytic and regulatory domains. The binding of Ca++/calmodulin to its regulatory domain releases its autoinhibitory effect and activates the kinase. The activated CaMKII further autophosphorylates itself at Thr286 to render the kinase constitutively active.
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