|Reactivity||Human, Mouse, Rat|
|Application & Usage||Western blotting at 1-2 µg/ml. However, the optimal concentrations should be determined individually. The anti-Phospho-CaMKII antibody recognizes only the phosphorylated CaMKII (Thr286) in samples from human, mouse, and rat origins. |
|Other Names||CAMK2A , CAMKA , KIAA0968, EC 188.8.131.52|
|Formulation||100 µl purified rabbit polyclonal anti-Phospho-CaMKII (Thr286) antibody in phosphate-buffered saline (PBS) containing 50% glycerol, 1% BSA, and 0.02% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||Phospho-CaMKII Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
CaMKII is a member of calcium/calmodulin-activated kinase, functioning in neural synaptic stimulation and T-cell receptor signaling. CaMKII has catalytic and regulatory domains. The binding of Ca++/calmodulin to its regulatory domain releases its autoinhibitory effect and activates the kinase. The activated CaMKII further autophosphorylates itself at Thr286 to render the kinase constitutively active.
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