|Application ||WB, IHC, IP|
|Reactivity||Human, Mouse, Rat, Rabbit, Hamster, Monkey|
|Calculated MW||32819 Da|
|Application & Usage||Western blotting (0.5-2 µg/ml), immunoprecipitation (10-20 µg/ml), and Immunohistochemistry (10-20 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes ~32-35 kDa Heme-Oxygenase-1. Jurkat cell lysate can be used as a positive control.|
|Other Names||HMOX1 , HO , HO-1 , HO1 , OTTHUMP00000028925 , bK286B10 , EC 188.8.131.52|
|Formulation||100 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||Heme Oxygenase-1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Exhibits cytoprotective effects since excess of free heme sensitizes cells to undergo apoptosis.|
|Cellular Location||Microsome. Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side|
|Tissue Location||Expressed at higher levels in renal cancer tissue than in normal tissue (at protein level)|
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Provided below are standard protocols that you may find useful for product applications.
Heme oxygenase-1 (HO-1) or HSP32 is the inducible isoform of heme oxygenase which catalyzes the NADPH, O2 and cytochrome P450 reductase dependent oxidation of heme to carbon monoxide, iron and biliverdin that is immediately reduced to bilirubin. To date, three heme oxygenase isoforms HO-1, HO-2 and HO-3 have been identified.
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