|Application ||WB, IHC, IP|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||47584 Da|
|Application & Usage||Western blot analysis (2-4 µg/ml). Per researchers feedback, the antibody also works well in Immunoprecipation (10-20 µg/ml), and Immunohistochemistry (20-40 µg/ml). However, the optimal conditions should be determined individually. Detects 55-60 kDa Bcl-3 of human, mouse, and rat origins. Other species have not been tested.|
|Other Names||BCL3 , D19S37 , BCL4|
|Formulation||100 µg (0.2 mg/ml) Protein G affinity purified rabbit anti-Bcl-3 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||Bcl-3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Contributes to the regulation of transcriptional activation of NF-kappa-B target genes. In the cytoplasm, inhibits the nuclear translocation of the NF-kappa-B p50 subunit. In the nucleus, acts as transcriptional activator that promotes transcription of NF-kappa-B target genes. Contributes to the regulation of cell proliferation (By similarity).|
|Cellular Location||Nucleus. Cytoplasm. Cytoplasm, perinuclear region. Note=Ubiquitination via 'Lys- 63'-linked ubiquitin chains is required for nuclear accumulation|
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Provided below are standard protocols that you may find useful for product applications.
Bcl-3, a proto-oncogene, is believed to be involved in certain human B cell leukemias. It encodes a protein that functions as an IkB-like molecule for native NFkB, but is specific for the p50 subunit of NFkB.
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