IRS-1 Antibody
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC |
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Primary Accession | P35568 |
Reactivity | Human, Mouse, Rat |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 131591 Da |
Gene ID | 3667 |
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Application & Usage | Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually |
Other Names | IRS1 , HIRS-1 |
Target/Specificity | IRS-1 |
Antibody Form | Liquid |
Appearance | Colorless liquid |
Formulation | 100 µg (0.5 mg/ml) purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA and 0.01% thimerosal. |
Handling | The antibody solution should be gently mixed before use. |
Reconstitution & Storage | -20 °C |
Background Descriptions | |
Precautions | IRS-1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | IRS1 |
---|---|
Function | May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit (By similarity). |
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Provided below are standard protocols that you may find useful for product applications.
Background
IRS-1 (Insulin receptor substrate-1) is phosphorylated in response to stimulation of cells by insulin. Upon ligand-induced phosphorylation, IRS-1 immediately associates with a series of SH2 domain-containing signaling intermediates such as PI3-kinase, GRB2, Syp and Nck. The level of diversity in structure and function of the SH2-containing proteins which binds activated IRS-1 is important for responsiveness to insulin. IRS phosphorylation elicits the growth and metablic effects of insulin growth factors and insulin by allowing glucose uptake, glycogen synthesis and mitogenic activity. IRS-1 migrates between 170 and 185 kDa and is expressed in most cell types.
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