|Application ||WB, IHC, IP|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||53455 Da|
|Application & Usage||Western blotting (1 µg/ml), immunoprecipitation (10-20 µg/ml), and Immunohistochemistry (10-20 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes p70 S6 kinase of human, mouse, and rat origins. Reactivity to other species has not been tested. |
|Other Names||RPS6KB1 , p70S6k , p70 , S6K , p70(S6K)-alpha , P70S6K , p70(S6K)-alpha , p70-alpha|
|Target/Specificity||p70 S6 Kinase|
|Formulation||100 µg (0.5 mg/ml) antigen affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 50% glycerol, 1% BSA, and 0.02% sodium azide.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||p70 S6 Kinase Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Phosphorylates specifically ribosomal protein S6.|
|Cellular Location||Cytoplasm. Nucleus.|
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Provided below are standard protocols that you may find useful for product applications.
p70 S6 kinase and p90 Rsk kinase are the two distinct 40S ribosomal protein S6 Ser/Thr kinases present in somatic animal cells. p90 Rsk kinase is maximally activated within minutes of addition of growth factors or phorbol ester to cultured cells followed by activation of p70 S6 kinase. Both enzymes are regulated by serine/threonine phosphorylation, s µggesting that specific kinases may exist upstream in the signaling pathways that regulate these kinases.
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