|Application ||WB, IHC|
|Calculated MW||11140 Da|
|Application & Usage||Western blotting (0.5-4 µg/ml) and Immunohistochemistry (10-20 µg/ml). However, the optimal conditions should be determined individually. Recognizes human cystatin B. Does not cross-react with cystatin A.|
|Other Names||Cystatin-B, CPI-B, Liver thiol proteinase inhibitor, Stefin-B, CSTB, CST6, STFB|
|Formulation||100 µg Protein G purified mouse anti-Cystatin B monoclonal antibody (mouse IgG1) in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||Cystatin B Antibody (Clone RJMW2E7) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||This is an intracellular thiol proteinase inhibitor. Tightly binding reversible inhibitor of cathepsins L, H and B.|
|Cellular Location||Cytoplasm. Nucleus|
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Provided below are standard protocols that you may find useful for product applications.
Cystatin B (stefin B, NCPI) is a natural inhibitor of lysosomal cysteine proteases (cathepsins). Its expression seems to be connected to the regulation of proteolysis which is essential for many physiological and pathological processes.
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