|Calculated MW||45538 Da|
|Application & Usage||Western blotting (0.5-4 µg/ml). It recognizes ~50 kDa FIAF from samples of mouse and rat origins.|
|Other Names||ANGPTL4 , ANGPTL-4 , UNQ171/PRO197 , FIAF , ARP4 , ANGPTL2 , Fasting- Induced Adipose Factor , NL2 , pp1158 , PSEC0166 , HFARP|
|Formulation||100 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||FIAF Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||Farp, Fiaf, Ng27|
|Function||Protein with hypoxia-induced expression in endothelial cells. May act as a regulator of angiogenesis and modulate tumorigenesis. Inhibits proliferation, migration, and tubule formation of endothelial cells and reduces vascular leakage. May exert a protective function on endothelial cells through an endocrine action. It is directly involved in regulating glucose homeostasis, lipid metabolism, and insulin sensitivity (By similarity). In response to hypoxia, the unprocessed form of the protein accumulates in the subendothelial extracellular matrix (ECM). The matrix-associated and immobilized unprocessed form limits the formation of actin stress fibers and focal contacts in the adhering endothelial cells and inhibits their adhesion. It also decreases motility of endothelial cells and inhibits the sprouting and tube formation.|
|Cellular Location||Secreted. Secreted, extracellular space, extracellular matrix. Note=The unprocessed form interacts with the extracellular matrix. This may constitute a dynamic reservoir, a regulatory mechanism of the bioavailability of ANGPTL4|
|Tissue Location||Predominantly expressed in adipose tissue and is strongly up-regulated by fasting in white adipose tissue and liver.|
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Provided below are standard protocols that you may find useful for product applications.
Adipose tissue is an energy reserve in animals. Adipose cells produce and secrete numerous physiologically important proteins that help regulate whole body metabolism, such as adiponectin leptin. Fasting causes significant changes in nutrient metabolism and many of these changes are controlled by transcription factors that regulate rate-limiting enzymes. An important transcription factor that mediates metabolic processes induced by fasting is peroxisome proliferator-activated receptor alpha (PPAR alpha). PPAR alpha has recently been reported to target a novel gene encoding the secreted protein FIAF (fasting-induced adipose factor). FIAF is expressed predominantly in adipose tissue and is strongly upregulated in white adipose tissue and the liver under fasting conditions. Initial studies s µggest that FIAF represents a novel endocrine signal helping to regulate metabolism, especially under fasting conditions.
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