|Application ||WB, IHC, IP|
|Reactivity||Human, Mouse, Rat, Chicken|
|Calculated MW||46534 Da|
|Application & Usage||Western blotting (0.5-4 µg/ml), immunoprecipitation (10-20 µg/ml) and Immunohistochemistry (10-20 µg/ml) in frozen or paraffin embedded tissues. However, the optimal concentrations should be determined individually. The antibody recognizes 47 kDa Hsp47 of human, mouse, rat, and chick origins. Mouse small intestine tissue lysate can be used as a positive control.|
|Other Names||SERPINH1 , AsTP3 , CBP1 , CBP2 , HSP47 , PIG14 , RA-A47 , PPROM , colligen , colligin-2 , SERPINH2|
|Formulation||100 µg (0.2 mg/ml) Protein A purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||Hsp47 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Binds specifically to collagen. Could be involved as a chaperone in the biosynthetic pathway of collagen.|
|Cellular Location||Endoplasmic reticulum lumen.|
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Provided below are standard protocols that you may find useful for product applications.
Heat shock proteins (HSPs) are ubiquitously expressed in all organisms. HSP47 is a molecule expressed constitutively in cells that synthesizes collagen. HSP47 is a procollagen-specific molecular chaperon that resides in the endoplasmic reticulum of procollagen-producing cells and is essential for secretion of procollagen from cells. Significantly, HSP47 plays a vital role in folding and assembling collagen. HSP47 is also known as colligin 1, serpinh1, collagen-binding protein 1 (CBP1) and gp46. The human HSP 47 gene encodes a 47 kDa protein.
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