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Lck Antibody

Rabbit Polyclonal Antibody

  • WB - Lck Antibody ABV10369
    Western blot analysis of Lck expression in rat kidney tissue lysate.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P06239
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 58001 Da
Additional Information
Gene ID 3932
Application & Usage Western blotting (0.5-4 µg/ml), immunoprecipitation (20-25 µg/ml), and Immunohistochemistry (20-25 µg/ml). However, the optimal conditions should be determined individually. The antibody detects 56 kDa Lck protein from samples of human, mouse, rat origins. A low band ~25 kDa can also be detected, presumably to be a cleavage fragment of Lck.
Other Names LCK , p56-LCK , p56lck , YT16 , pp58lck , LSK
Target/Specificity Lck
Antibody Form Liquid
Appearance Colorless liquid
Formulation 100 µg (0.5 mg/ml) antigen affinity purified rabbit polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
Handling The antibody solution should be gently mixed before use.
Reconstitution & Storage -20 °C
Background Descriptions
PrecautionsLck Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name LCK
Function Non-receptor tyrosine-protein kinase that plays an essential role in the selection and maturation of developing T- cells in the thymus and in the function of mature T-cells. Plays a key role in T-cell antigen receptor (TCR)-linked signal transduction pathways. Constitutively associated with the cytoplasmic portions of the CD4 and CD8 surface receptors. Association of the TCR with a peptide antigen-bound MHC complex facilitates the interaction of CD4 and CD8 with MHC class II and class I molecules, respectively, thereby recruiting the associated LCK protein to the vicinity of the TCR/CD3 complex. LCK then phosphorylates tyrosines residues within the immunoreceptor tyrosine-based activation motifs (ITAM) of the cytoplasmic tails of the TCR-gamma chains and CD3 subunits, initiating the TCR/CD3 signaling pathway. Once stimulated, the TCR recruits the tyrosine kinase ZAP70, that becomes phosphorylated and activated by LCK. Following this, a large number of signaling molecules are recruited, ultimately leading to lymphokine production. LCK also contributes to signaling by other receptor molecules. Associates directly with the cytoplasmic tail of CD2, which leads to hyperphosphorylation and activation of LCK. Also plays a role in the IL2 receptor-linked signaling pathway that controls the T-cell proliferative response. Binding of IL2 to its receptor results in increased activity of LCK. Is expressed at all stages of thymocyte development and is required for the regulation of maturation events that are governed by both pre-TCR and mature alpha beta TCR. Phosphorylates other substrates including RUNX3, PTK2B/PYK2, the microtubule-associated protein MAPT, RHOH or TYROBP.
Cellular Location Cytoplasm. Cell membrane; Lipid-anchor; Cytoplasmic side. Note=Present in lipid rafts in an inactive form
Tissue Location Expressed specifically in lymphoid cells.
Research Areas
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Lck is a membrane associated tyrosine protein kinases. The Lck gene has been shown to undergo rearrangement and overexpression in some murine lymphomas. In human studies, it has been demonstrated that the Lck gene is localized to a site in the genome which undergoes frequent chromosomal abnormalities in lymphomas and neuroblastomas. Resting T cells contain high levels of the Lck protein and mRNA both of which decline upon activation of the T cells. These and other observations s µggest that alterations in Lck expression may contribute to the pathogenesis of some types of neoplastic disease.

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$ 245.00
Cat# ABV10369
(40 western blots)
Availability: 5-7days
Bulk Size
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