|Application ||WB, IHC|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||35676 Da|
|Application & Usage||Western blot analysis (1 µg/ml) and Immunohistochemistry (10-20 µg/ml). However, the optimal conditions should be determined individually. The Phospho-c-Jun (Ser73) antibody detects only activated c-Jun when phosphorylated at Ser73. It does not react with non-phosphorylated c-Jun. |
|Other Names||JUN , AP1 , p39 , c-Jun|
|Formulation||100 µg (0.5 mg/ml) affinity-purified rabbit polyclonal phospho-c-Jun (Ser73) antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||Phospho-c-Jun (Ser73) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation.|
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Provided below are standard protocols that you may find useful for product applications.
c-Jun is a component of the transcription factor AP-1 that binds and activates transcription at TRE/AP-1 elements. The transcriptional activity of c-Jun is regulated by phosphorylation at Ser63 and Ser73. Extracellular signals, such as growth factors and UV irradiation, stimulate phosphorylation of c-Jun at Ser63/73 and activate c-Jun dependent transcription. The MAP kinase homologue, SAPK/JNK, binds to the N-terminal region of c-Jun and phosphorylates c-Jun at Ser63/73.
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