|Calculated MW||54002 Da|
|Application & Usage||Western blotting (1-4 µg/ml). However, the optimal concentrations should be determined individually.The antibody recognizes a ~50 kDa band from samples of human origin. Reactivity to other species has not been tested. Jurkat cell lysate can be used as a positive control.|
|Other Names||HME; MME , Matrix metalloproteinase|
|Formulation||100 µg (0.2 mg/ml) protein A purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 50% glycerol, 1% BSA, and 0.02% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||MMP-12 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3.|
|Cellular Location||Secreted, extracellular space, extracellular matrix|
|Tissue Location||Found in alveolar macrophages but not in peripheral blood monocytes|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
email@example.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including collagen, gelatin, fibronectin, laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB). MMP catalysis requires both calcium and zinc. MMP-12 (also designated macrophage metalloelastase) is produced in alveolar macrophages and degrades elastin. MMP-12 may contribute to elastin degradation occurring in granulomatous skin diseases and may also participate in macrophage migration thro µgh the epidermal and vascular basement membranes in inflammatory disorders.
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at firstname.lastname@example.org.