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NSF Antibody

Rabbit Polyclonal Antibody

     
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IP
Primary Accession P46460
Reactivity Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 82613 Da
Additional Information
Gene ID 18195
Application & Usage Western blotting (1-2 µg/ml), immunoprecipitation (5-15 mg/ml) and Immunohistochemistry (10-20 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes 85 kDa NSF of mouse, and rat origins. Reactivity to other species has not been tested
Other Names SKD2 , N-ethylmaleimide-sensitive Fusion Protein , Anti-NSF, NSF
Target/Specificity NSF
Antibody Form Liquid
Appearance Colorless liquid
Formulation 100 µg (0.5 mg/ml) Protein A purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 50% glycerol, 1% BSA, and 0.02% sodium azide.
Handling The antibody solution should be gently mixed before use.
Reconstitution & Storage -20 °C
Background Descriptions
PrecautionsNSF Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Nsf
Synonyms Skd2
Function Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seems to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack GRIA2 leads to influence GRIA2 membrane cycling (By similarity).
Cellular Location Cytoplasm.
Research Areas
Citations (0)
citation

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Background

Syntaxins were originally tho µght to be docking proteins, but have more recently been categorized as anchoring proteins that anchor themselves to the cytoplasmic surfaces of cellular membranes. Syntaxins have been shown to bind to various proteins involved in exocytosis, including VAMPs (vesicle-associated membrane proteins), NSF (N-ethylmaleimidesensitive factor), SNAP 25 (synaptosomal-associated protein of 25kDa), SNAPs (soluble NSF attachment proteins) and synaptotagmin. SNAPs mediate the membrane binding of NSF, which is essential for membrane fusion reactions. An additional protein designated synaptophysin may regulate exocytosis by competing with SNAP 25 and syntaxins for VAMP binding.

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Discontinued
Cat# ABV10396-30
Size:

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