|Reactivity||Human, Mouse, Rat, Monkey|
|Calculated MW||89449 Da|
|Application & Usage||Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The antibody recognizes ~86 kDa TLR2 in Jurkat cell lysate. Reactivity to other species has not been tested. Blocking peptide is available separately.|
|Other Names||TLR2 , Anti-TLR2 , CD282 , TIL4, Toll-like receptor 2|
|Formulation||100 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||TLR2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 or TLR6 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. May also promote apoptosis in response to lipoproteins (By similarity).|
|Cellular Location||Cell membrane; Single-pass type I membrane protein. Cytoplasmic vesicle, phagosome membrane; Single-pass type I membrane protein|
|Tissue Location||Detected in a macrophage cell line, smooth muscle, lung, spleen, thymus, brain and adipose tissue|
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Provided below are standard protocols that you may find useful for product applications.
The Toll-like receptor (TLR) family in mammalian comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Up-to-date, ten TLRs (TLR1-10) have been described. TLR2 is differentially expressed in human cells. CD14+ monocytes expressed the highest level of TLR2 followed by CD15+ granulocytes, and CD19+ B-cells. CD3+ T-cells and CD56+ NK cells did not express TLR2. The expression of TLR2 on different cell types is regulated by different immune response modifiers. For example, LPS, GM-CSF, IL-1, and IL-10 up regulate TLR2, whereas IL-4, IFN-gamma, and TNF down regulate TLR2 expression in monocytes.
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