|Reactivity||Human, Mouse, Rat|
|Calculated MW||97627 Da|
|Application & Usage||Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. Other applications have not been determined. The antibody recognizes ~97 kDa TLR5 in sample from human, mouse and rat origins. Reactivity to other species has not been tested. Blocking peptide is available separately.|
|Other Names||TLR5 , Anti-TLR5 , FLJ10052 , MGC126430 , MGC126431 , TLR 5|
|Formulation||100 µg (0.5 mg/ml) affinity purified rabbit anti-TLR5 polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||TLR5 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Participates in the innate immune response to microbial agents. Mediates detection of bacterial flagellins. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (By similarity).|
|Cellular Location||Membrane; Single-pass type I membrane protein|
|Tissue Location||Highly expressed in liver. Detected in lung and at very low levels in most other tissues|
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Provided below are standard protocols that you may find useful for product applications.
The Toll-like receptor (TLR) family of proteins are characterized by a highly conserved Toll homology (TH) domain, which is essential for Toll-induced signal transduction. TLR1, as well as the other TLR family members, are type I transmembrane receptors that characteristically contain an extracellular domain consisting of several leucine-rich regions along with a single cytoplasmic Toll/IL-1R-like domain. TLR2 and TLR4 are activated in response to lipopolysacchride (LPS) stimulation, which results in the activation and translocation of NFkB and s µggests that these receptors are involved in mediating inflammatory responses. TLR6 is highly homologous to TLR1, and like other members of TLR family, it induces NFkB signaling upon activation. Expression of TLR receptors is highest in peripheral blood leukocytes, macrophages, and monocytes.
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