|Reactivity||Human, Mouse, Rat|
|Calculated MW||39656 Da|
|Application & Usage||Western blotting (0.5-4 µg/ml), Immunohistochemistry (10-20 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes 40-46 kDa Wnt-6 from rat origins. Reactivity to other species has not been tested.|
|Other Names||wnt6, wnt 6, wnt-6, wingless-related MMTV integration site 6,|
|Formulation||100 µg (0.5 mg/ml) affinity purified rabbit Wnt-6 polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||Wnt-6 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.|
|Cellular Location||Secreted, extracellular space, extracellular matrix|
|Tissue Location||Detected in ileum, colon and stomach (at protein level).|
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Provided below are standard protocols that you may find useful for product applications.
Wnt gene family members, including Wnt-1 thro µgh Wnt-10, play a key role in regulating cellular growth and differentiation. Wnt-1 is a cysteine-rich, secreted glycoprotein that associates with cell membranes and likely functions as a key regulator of cellular adhesion. Wnt-1, which is essential for normal development of the embryonic nervous system, contributes to hyperplasia and tumorigenic progression when improperly expressed in mammary tissue. Wnt-3 is involved in tumorigenesis and Wnt-2 and Wnt-4 may be associated with abnormal proliferation in human breast tissue. Wnt-6 is involved in oncogenesis and is co-expressed with Wnt10 in colorectal cancer cell lines. The presence of cervical and breast cell carcinomas may indicate an over expression of Wnt-6.
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