|Reactivity||Human, Mouse, Rat|
|Calculated MW||72232 Da|
|Application & Usage||Western blotting (1-4 µg/ml). However, the optimal conditions should be determined individually. The antibody detects 70 kDa LIMK2 protein. It does not cross-react with LIMK1.|
|Other Names||EC 188.8.131.52 , LIMK-2 , LIM domain kinase 2 , LIMK|
|Formulation||100 µg (0.5 mg/ml) peptide affinity purified rabbit polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||LIMK2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Displays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in vitro.|
|Cellular Location||Isoform LIMK2a: Cytoplasm. Nucleus. Note=Isoform LIMK2a is distributed in the cytoplasm and the nucleus|
|Tissue Location||Highest expression in the placenta; moderate level in liver, lung, kidney, and pancreas. LIMK2a is found to be more abundant then LIMK2b in liver, colon, stomach, and spleen, while in brain, kidney, and placenta LIMK2b is the dominant form In adult lung, both LIMK2a and LIMK2b is nearly equally observed|
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Provided below are standard protocols that you may find useful for product applications.
LIM kinases (LIMK1 and LIMK2) are serine/threnine kinases that have two zinc finger motifs, known as LIM motifs in their N-terminal regulatory domain. LIM kinases are involved in actin cytoskeleton regulation thro µgh Rho-family GTPases and downstream kinases PAKs and ROCK. PAK1 and ROCK phosphorylate LIMK1 and LIMK2, which increases the activity of the kinases. Activated LIM kinases inhibit the actin depolymerization activity of cofilin by phosphorylation at the N-terminus of Cofilin.
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