|Application ||WB, IHC|
|Reactivity||Human, Mouse, Rat, Rabbit, Bovine|
|Calculated MW||30773 Da|
|Positive Control||Western Blot: 3T3 cell lysate. IHC: Liver tissue|
|Application & Usage||Western blotting (0.5-4 µg/ml) and Immunohistochemistry (2.5 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes ~31 kDa VDAC/Porin from samples of human, mouse, rat, bovine, pig, and rabbit origins.|
|Other Names||VDAC1, VDAC-1 , MGC111064 , hVDAC1, Porin|
|Formulation||100 µg (0.5 mg/ml) affinity purified rabbit anti-VDAC/Porin polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||VDAC/Porin Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Forms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective (PubMed:11845315, PubMed:18755977, PubMed:20230784, PubMed:8420959). May participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis (PubMed:15033708, PubMed:25296756).|
|Cellular Location||Mitochondrion outer membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein Membrane raft; Multi-pass membrane protein|
|Tissue Location||Heart, liver and skeletal muscle.|
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Provided below are standard protocols that you may find useful for product applications.
The Voltage-Dependent Anion Channel (VDAC or mitochondrial Porin)I is an outer membrane mitochondrial protein. The VDAC protein is tho µght to form the major pores thro µgh which adenine nucleotides are transferred thro µgh the outer mitochondrial membrane. VDAC has also been implicated in the formation of the mitochondrial permeability transition pore complex in apoptotic cells. This complex, formed by VDAC, ANT, and CypD is tho µght to allow the mitochondria to undergo metabolic uncoupling and irreversible morphologic changes that ultimately destroy the mitochondria during apoptosis.
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