|Application ||WB, ICC|
|Reactivity||Human, Mouse, Rat, Hamster, Chicken, Dog|
|Calculated MW||55364 Da|
|Application & Usage||Western blotting (0.5-4 µg/ml) and immunocytochemistry (10-20 µg/ml). However, the optimal conditions should be determined individually. The antibody detects 55 kDa Histone Deacetylase 2 in samples from human, mouse, rat, chicken, hamster, and canine origins.|
|Other Names||HDAC-2, Histone Deacetylase 2, RPD3, YAF1|
|Formulation||100 µg (0.2 mg/ml) affinity purified rabbit anti-HDAC2 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||HDAC2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Forms transcriptional repressor complexes by associating with MAD, SIN3, YY1 and N-COR. Interacts in the late S-phase of DNA-replication with DNMT1 in the other transcriptional repressor complex composed of DNMT1, DMAP1, PCNA, CAF1. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. May be involved in the transcriptional repression of circadian target genes, such as PER1, mediated by CRY1 through histone deacetylation. Involved in MTA1-mediated transcriptional corepression of TFF1 and CDKN1A.|
|Cellular Location||Nucleus. Cytoplasm|
|Tissue Location||Widely expressed; lower levels in brain and lung|
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Provided below are standard protocols that you may find useful for product applications.
HDAC2 is a class I mammalian histone deacetylase containing 488 amino acid residues with an apparent size of ~55 kDa by denaturing SDS-PAGE. HDAC2 has been shown to interact directly with transcription repressor complexes and the nuclear receptor corepressor N-CoR.
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