|Reactivity||Human, Mouse, Rat|
|Calculated MW||111297 Da|
|Application & Usage||Western blotting (0.5-4 µg/ml), Immunoprecipitation and immunofluorescence. However, the optimal concentrations should be determined individually. The antibody recognizes a ~110-140 kDa of HDAC-9. A 75 kDa and a 60 kDa bands can also be detected in some tissue lysates.|
|Other Names||HD9 , Histone deacetylase 9|
|Formulation||100 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||HDAC9 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||HDAC7, HDAC7B, HDRP, KIAA0744, MITR|
|Function||Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Represses MEF2-dependent transcription.|
|Tissue Location||Broadly expressed, with highest levels in brain, heart, muscle and testis. Isoform 3 is present in human bladder carcinoma cells (at protein level)|
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Provided below are standard protocols that you may find useful for product applications.
HDAC family members have been divided into two classes. Class I of the HDACs comprises four members, HDAC-1, 2, 3, and 8, each of which contains a deacetylase domain exhibiting from 45 to 93% identity in amino acid sequence. Class II of the HDACs comprises HDAC-4, 5, 6, and 7, the molecular weights of which are all about two-fold larger than those of the class I members, and the deacetylase domains are present within the C-terminal regions, except that HDAC-6 contains two copies of the domain, one within each of the N-terminal and C-terminal regions. Human HDAC-1, 2 and 3 were expressed in various tissues, but the others (HDAC-4, 5, 6, and 7) showed tissue-specific expression patterns.
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