|Application ||WB, IF, IP|
|Calculated MW||18172 Da|
|Application & Usage||The antibody can be used for Western blotting (1:1000), Immunofluorescence (1:1000) and Immunohistochemistry (1:1000). However, the optimal conditions should be determined individually.|
|Other Names||Thy-1 membrane glycoprotein, Thy-1 antigen, CD90, Thy1, Thy-1|
|Target/Specificity||Phospho (Ser19) THY|
|Formulation||100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||Phospho (Ser19) Tyrosine Hydroxylase Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.|
|Cellular Location||Cell membrane; Lipid-anchor, GPI-anchor.|
|Tissue Location||Abundant in lymphoid tissues.|
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Provided below are standard protocols that you may find useful for product applications.
Tyrosine hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines Dopamine and Norepinephrine. TH antibodies can therefore be used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson’s disease and dr µg abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999). TH antibodies can also be used to explore basic mechanisms of dopamine and norepinephrine signaling (Witkovsky et al., 2000; Salvatore et al., 2001; Dunkley et al., 2004). The activity of TH is also regulated by phosphorylation (Haycock et al., 1982; Haycock et al., 1992; Jedynak et al., 2002). Phospho-specific antibodies for the phosphorylation sites on TH can be used to great effect in studying this regulation and in identifying the cells in which TH phosphorylation occurs.
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