|Application ||IHC, IF, FC, ICC, E, IP|
|Application & Usage||The antibody can be used in IHC, IP, ICC, ELISA, IF, FACS. However, the optimal concentrations should be determined individually.|
|Other Names||BrdU, BUdR, BrdUrd|
|Formulation||100 µl (0.5 mg/ml) in 10% glycerol in PBS.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-80°C|
|Precautions||Anti-BrdU antibody (Clone B1G5) is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU, BUdR, BrdUrd) is a synthetic nucleoside that is an analogue of thymidine. The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthesized DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis. Antibody specific for BrdU is used to immunocytochemically detect the incorporated BrdU, which is a powerful tool to study cell proliferation, the cytokinetics of normal and neoplastic cells, etc.
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