|Application ||WB, IHC, IP|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||14105 Da|
|Application & Usage||Western blotting (0.5-4 µg/ml), immunoprecipitation (10-20 µg/ml) and Immunohistochemistry (10-30 µg/ml). However, the optimal conditions should be determined individually. The antibody detects ~14 kDa histone H2A protein. It does not cross-react with other histones. HeLa cell nuclear extract can be used as a positive control.|
|Other Names||H2AFX , H2A/X , H2A.X , H2AX , H2a/x|
|Formulation||100 µg (0.5 mg/ml) affinity purified rabbit anti-Histone H2A polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||Histone H2A Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.|
|Cellular Location||Nucleus. Chromosome.|
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Provided below are standard protocols that you may find useful for product applications.
The nucleosome is made up of four core histone proteins (H2A, H2B, H3 and H4) and is the primary building block of chromatin. The N-terminal tail of core histones undergoes different posttranscriptional modification including acetylation, phosphorylation and methylation. These modifications occur in response to cell signal stimuli and have a direct effect on gene expression.
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