|Application ||WB, IHC, IP|
|Reactivity||Human, Mouse, Rat|
|Application & Usage||Western blotting (0.5-4 µg/ml), in immunoprecipitation (20-40 µg/ml) and Immunohistochemistry (20-40 µg/ml). However, the optimal conditions should be determined individually. The antibody detects ~11 kDa histone H4 protein. It does not cross-react with other histones. |
|Other Names||H4FB , HIST1H4B , HIST1H4F, H4FN, H4FH|
|Formulation||100 µg (0.5 mg/ml) affinity purified rabbit anti-Histone H4 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||Histone H4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
The nucleosome is made up of four core histone proteins (H2A, H2B, H3 and H4) and is the primary building block of chromatin. The N-terminal tail of core histones undergoes different posttranscriptional modification including acetylation, phosphorylation and methylation. These modifications occur in response to cell signal stimuli and have a direct effect on gene expression.
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