|Application ||WB, IHC|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||83099 Da|
|Application & Usage||Western blotting (0.5-4 µg/ml). However, the optimal concentrations should be determined individually. Jurkat cell lysate can be used as a positive control.|
|Other Names||SELPLG , CLA , PSGL-1 , CD162 , PSGL1|
|Formulation||100 µg (0.5mg/ml) affinity purified rabbit anti-P-Selectin polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||P-Selectin Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1.|
|Cellular Location||Membrane; Single-pass type I membrane protein|
|Tissue Location||Stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface|
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Provided below are standard protocols that you may find useful for product applications.
Selectins are a family of carbohydrate-binding proteins involved in mediating cellular interactions with leukocytes. E-Selectin (also called ELMA-1) expressed on endothelial cells. L-Selectin (also called LECAM-1) is expressed on the majority of B and naïve T cells and on most monocytes, neurophils and eosinophils. P-Selectin (also called GMP-140) expressed on activated platelets and endothelial cells. Selectins interact with specific carbohydrates expressed by activated endothelial cells, monocytes, and neutrophils.
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