P-Selectin Antibody
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC |
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Primary Accession | Q01102 |
Reactivity | Human, Mouse, Rat |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 83099 Da |
Gene ID | 20344 |
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Application & Usage | Western blotting (0.5-4 µg/ml). However, the optimal concentrations should be determined individually. Jurkat cell lysate can be used as a positive control. |
Other Names | SELPLG , CLA , PSGL-1 , CD162 , PSGL1 |
Target/Specificity | P-Selectin |
Antibody Form | Liquid |
Appearance | Colorless liquid |
Formulation | 100 µg (0.5mg/ml) affinity purified rabbit anti-P-Selectin polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal. |
Handling | The antibody solution should be gently mixed before use. |
Reconstitution & Storage | -20 °C |
Background Descriptions | |
Precautions | P-Selectin Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | Selp |
---|---|
Synonyms | Grmp |
Function | Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with SELPLG. |
Cellular Location | Cell membrane {ECO:0000250|UniProtKB:P16109}; Single-pass type I membrane protein {ECO:0000250|UniProtKB:P16109} |
Tissue Location | Stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface |
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Provided below are standard protocols that you may find useful for product applications.
Background
Selectins are a family of carbohydrate-binding proteins involved in mediating cellular interactions with leukocytes. E-Selectin (also called ELMA-1) expressed on endothelial cells. L-Selectin (also called LECAM-1) is expressed on the majority of B and naïve T cells and on most monocytes, neurophils and eosinophils. P-Selectin (also called GMP-140) expressed on activated platelets and endothelial cells. Selectins interact with specific carbohydrates expressed by activated endothelial cells, monocytes, and neutrophils.
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