|Reactivity||Human, Mouse, Rat, Chicken, Bovine|
|Calculated MW||53652 Da|
|Application & Usage||Western blotting (0.5-4 µg/ml), immunoprecipitation (10-20 µg/ml) and Immunohistochemistry (10-20 µg/ml). However, the optimal concentrations should be determined individually.|
|Other Names||VIM , FLJ36605 , Vimentin , vimentin|
|Formulation||100 µg (0.2mg/ml) rabbit anti-Vimentin polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA and 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||Vimentin Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.|
|Tissue Location||Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone- independent mammary carcinoma cell lines|
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Provided below are standard protocols that you may find useful for product applications.
Vimentin, a intermediate filament protein, is a general marker of cells originating in the mesenchyme. Vimentin is frequently co-expressed with other members of the intermediate filament family such as the cytokeratins, in neoplasm.
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