|Application ||WB, IHC|
|Positive Control||Western Blot: Jurkat cell lysate. IHC: Placenta tissue|
|Application & Usage||Western blotting (0.5-4 µg/ml) and Immunohistochemistry (5 µg/ml) . However, the optimal conditions should be determined individually. Jurkat cell lysate can be used as a positive control. The antibody detects 36 kDa annexin VIII of human origin. The ~28-30 kDa cleavage fragments can also be detected in Jurkat cell lysate|
|Other Names||ANXA7 , ANX7 , SNX , Annexin A7|
|Formulation||100 µg (0.5 mg/ml) protein A purified rabbit anti-Annexin VIII polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||Annexin VIII Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
The annexin family of calcium-binding proteins is composed of at least ten mammalian genes. It is characterized by a conserved core domain which binds to phospholipids in a Ca2+-dependent manner. Annexin family members have been implicated as regulators of diverse processes as ion flux, endocytosis and exocytosis, and cellular adhesion. Annexin VIII is preferentially expressed in acute promyelocytic leukemia (APL) cells which may relate its role in hematopoietic cell differentiation.
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