|Application ||WB, ICC, IP|
|Application & Usage||Western blotting (1-2 µg/ml), immunoprecipitation (10-20 µg/ml), and immunocytochemistry (10-20 µg/ml). However, the optimal conditions should be determined individually.|
|Other Names||Anti His-tag|
|Formulation||100 µg (0.5 mg/ml) mouse anti-His-Tag monoclonal antibody in phosphate buffered saline (PBS, pH 7.2), containing 50% glycerol, 1% BSA, 0.02% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||His-Tag Antibody (Clone BV-G020) is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
It is expected that the His-Tag monoclonal antibody will react with proteins tagged with His from all species.
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