|Application ||WB, IHC|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||41709 Da|
|Positive Control||Western Blot: Jurkat cell lysate and mouse small intestine tissue. IHC: Liver tissue|
|Application & Usage||Western blotting (0.5-4 µg/ml), and Immunohistochemistry (5 µg/ml). However, the optimal conditions should be determined individually. Jurkat cell lysate can be used as positive controls. The antibody detects the full length and cleaved form of Cyr61 protein from samples of human, mouse and rat origins. Cross-reactivity to other species has not been determined.|
|Other Names||CYR61 , 602369 , O00622 , CCN1 , GIG1 , IGFBP10|
|Formulation||100 µg (0.5 mg/ml) affinity purified rabbit anti-Cyr61 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||Cyr61 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Promotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up- regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5 (By similarity). CYR61-mediated gene regulation is dependent on heparin-binding (By similarity). Down- regulates the expression of alpha-1 and alpha-2 subunits of collagen type-1 (By similarity). Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-1/beta-5 and cell proliferation through integrin alpha-v/beta-3 (By similarity).|
|Tissue Location||Low in kidney, adrenal gland, testes, brain, and ovary, moderate in heart, uterus, and skeletal muscle, highest in lung|
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Provided below are standard protocols that you may find useful for product applications.
Cyr61 is a member of angiogenic and vasculogenic regulators designated CCN proteins, which includes CTGF, Cyr61 homolog, Fisp2. Cyr61 functions as an angiogenic inducer, stimulating cell adhesion and migration and promotes DNA synthesis of human vascular endothelial cells. Elevated expression of Cyr61 is observed during vessel growth, wound healing and chondrocyte differentiation.
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