Phospho-Tyrosine Hydroxylase Antibody
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC, IF |
---|---|
Primary Accession | P07101 |
Other Accession | AAI04968 |
Reactivity | Human, Mouse, Rat |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 58600 Da |
Gene ID | 7054 |
---|---|
Application & Usage | Western blotting (1:200-1000) and immunofluorescence (1:50-100). However, the optimal conditions should be determined individually. Other applications have not been determined. The antibody detects a ~60 kDa Tyrosine Hydroxylase when phosphorylated at Ser40. |
Other Names | Tyrosin Hydroxylase |
Target/Specificity | Phospho-Tyrosin Hydroxylase |
Antibody Form | Liquid |
Appearance | Colorless liquid |
Formulation | 100 µl antigen affinity purified rabbit anti-phospho-Tyrosine Hydroxylase polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% Thimerosal. |
Handling | The antibody solution should be gently mixed before use. |
Reconstitution & Storage | -20 °C |
Background Descriptions | |
Precautions | Phospho-Tyrosine Hydroxylase Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | TH (HGNC:11782) |
---|---|
Synonyms | TYH |
Function | Catalyzes the conversion of L-tyrosine to L- dihydroxyphenylalanine (L-Dopa), the rate-limiting step in the biosynthesis of cathecolamines, dopamine, noradrenaline, and adrenaline. Uses tetrahydrobiopterin and molecular oxygen to convert tyrosine to L-Dopa (PubMed:17391063, PubMed:1680128, PubMed:15287903, PubMed:8528210, Ref.18, PubMed:34922205, PubMed:24753243). In addition to tyrosine, is able to catalyze the hydroxylation of phenylalanine and tryptophan with lower specificity (By similarity). Positively regulates the regression of retinal hyaloid vessels during postnatal development (By similarity). |
Cellular Location | Cytoplasm, perinuclear region {ECO:0000250|UniProtKB:P24529}. Nucleus {ECO:0000250|UniProtKB:P04177} Cell projection, axon {ECO:0000250|UniProtKB:P24529}. Cytoplasm {ECO:0000250|UniProtKB:P04177}. Cytoplasmic vesicle, secretory vesicle, synaptic vesicle {ECO:0000250|UniProtKB:P04177}. Note=When phosphorylated at Ser-19 shows a nuclear distribution and when phosphorylated at Ser-31 as well at Ser-40 shows a cytosolic distribution (By similarity). Expressed in dopaminergic axons and axon terminals. {ECO:0000250|UniProtKB:P04177} |
Tissue Location | Mainly expressed in the brain and adrenal glands. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
info@abcepta.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Background
Tyrosine hydroxylase play an important role in the synthesis of dopamine and other catecholamines. The enzyme’s activity is regulated in the level of transcription and translation, as well as posttranscriptional modifications. Phosphorylation at Ser40 may play a key role in regulation of the catalytic activity of the enzyme.
If you have used an Abcepta product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abcepta.com.