|Application ||WB, IHC, IF|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||58600 Da|
|Application & Usage||Western blotting (1:200-1000) and immunofluorescence (1:50-100). However, the optimal conditions should be determined individually. Other applications have not been determined. The antibody detects a ~60 kDa Tyrosine Hydroxylase when phosphorylated at Ser40.|
|Other Names||Tyrosin Hydroxylase|
|Formulation||100 µl antigen affinity purified rabbit anti-phospho-Tyrosine Hydroxylase polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% Thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||Phospho-Tyrosine Hydroxylase Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Plays an important role in the physiology of adrenergic neurons.|
|Tissue Location||Mainly expressed in the brain and adrenal glands|
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Provided below are standard protocols that you may find useful for product applications.
Tyrosine hydroxylase play an important role in the synthesis of dopamine and other catecholamines. The enzyme’s activity is regulated in the level of transcription and translation, as well as posttranscriptional modifications. Phosphorylation at Ser40 may play a key role in regulation of the catalytic activity of the enzyme.
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