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Phospho-MARCKS Antibody
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB |
|---|---|
| Primary Accession | P30009 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 29795 Da |
| Application & Usage | Western blotting (1:200-1000). However, the optimal conditions should be determined individually. Other applications have not been determined. The antibody detects 80 kDa MARCKS only when phosphorylated at Ser152/156. |
|---|---|
| Other Names | MACS , PRKCSL , MRACKS , PKCSL , FLJ14368 , FLJ90045 , phosphomyristin |
| Target/Specificity | Phospho-MARCKS |
| Antibody Form | Liquid |
| Appearance | Colorless liquid |
| Formulation | 100 µl affinity purified rabbit anti-phospho-MARCKS polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% thimerosal. |
| Handling | The antibody solution should be gently mixed before use. |
| Reconstitution & Storage | -20 °C |
| Background Descriptions | |
| Precautions | Phospho-MARCKS Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | Marcks |
|---|---|
| Synonyms | Macs |
| Function | Membrane-associated protein that plays a role in the structural modulation of the actin cytoskeleton, chemotaxis, motility, cell adhesion, phagocytosis, and exocytosis through lipid sequestering and/or protein docking to membranes (PubMed:24662485). Thus, exerts an influence on a plethora of physiological processes, such as embryonic development, tissue regeneration, neuronal plasticity, and inflammation. Sequesters phosphatidylinositol 4,5-bisphosphate (PIP2) at lipid rafts in the plasma membrane of quiescent cells, an action reversed by protein kinase C, ultimately inhibiting exocytosis. During inflammation, promotes the migration and adhesion of inflammatory cells and the secretion of cytokines such as tumor necrosis factor (TNF), particularly in macrophages. Plays an essential role in bacteria- induced intracellular reactive oxygen species (ROS) formation in the monocytic cell type. Participates in the regulation of neurite initiation and outgrowth by interacting with components of cellular machinery including CDC42 that regulates cell shape and process extension through modulation of the cytoskeleton (By similarity). Plays also a role in axon development by mediating docking and fusion of RAB10-positive vesicles with the plasma membrane (PubMed:24662485). |
| Cellular Location | Cell membrane; Lipid-anchor. Cytoplasm, cytoskeleton {ECO:0000250|UniProtKB:P29966}. Cytoplasm {ECO:0000250|UniProtKB:P29966}. Note=PKC-dependent phosphorylation displaces MARCKS from the cell membrane and subsequent dephosphorylation is accompanied by its reassociation with the membrane. {ECO:0000250|UniProtKB:P29966} |
| Tissue Location | Highest levels found in spleen and brain. Intermediate levels seen in thymus, ovary, lung and heart. Very low levels seen in kidney, skeletal muscle and liver |
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Provided below are standard protocols that you may find useful for product applications.
Background
MARCKS (Myristoylated alanine-rich protein kinase C substrate) is a major PKC substrate that is distributed in various cell types. MARCKS has been implicated in cell motility, cell adhesion, phagocytosis, membrane traffic and mitogenesis. PKC phosphorylates Ser152, 156 and 163 of MARCKS, which regulates MARCKS's calcium/calmodulin binding activity and filamentous (F)-actin cross-linking activity. In addition, phosphorylation by PKC results in translocation of MARCKS from membrane to cytoplasm.
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