|Reactivity||Human, Mouse, Rat|
|Calculated MW||29795 Da|
|Application & Usage||Western blotting (1:200-1000). However, the optimal conditions should be determined individually. Other applications have not been determined. The antibody detects 80 kDa MARCKS only when phosphorylated at Ser152/156.|
|Other Names||MACS , PRKCSL , MRACKS , PKCSL , FLJ14368 , FLJ90045 , phosphomyristin|
|Formulation||100 µl affinity purified rabbit anti-phospho-MARCKS polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||Phospho-MARCKS Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.|
|Cellular Location||Cytoplasm, cytoskeleton. Membrane; Lipid-anchor|
|Tissue Location||Highest levels found in spleen and brain. Intermediate levels seen in thymus, ovary, lung and heart. Very low levels seen in kidney, skeletal muscle and liver|
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Provided below are standard protocols that you may find useful for product applications.
MARCKS (Myristoylated alanine-rich protein kinase C substrate) is a major PKC substrate that is distributed in various cell types. MARCKS has been implicated in cell motility, cell adhesion, phagocytosis, membrane traffic and mitogenesis. PKC phosphorylates Ser152, 156 and 163 of MARCKS, which regulates MARCKS's calcium/calmodulin binding activity and filamentous (F)-actin cross-linking activity. In addition, phosphorylation by PKC results in translocation of MARCKS from membrane to cytoplasm.
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