|Calculated MW||90472 Da|
|Positive Control||Jurkat cell lysate|
|Application & Usage||The antibody can be used in Western Blot analysis (1-4 µg/ml). However, the optimal concentrations should be determined individually. Blocking peptide is available separately.|
|Other Names||EGF-like module receptor 2, CD312|
|Formulation||100 µg (0.5 mg/ml) affinity purified rabbit anti- EMR2polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 5 mM EDTA and 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||EMR2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Cell surface receptor that binds to the chondroitin sulfate moiety of glycosaminoglycan chains and promotes cell attachment. Promotes granulocyte chemotaxis, degranulation and adhesion. In macrophages, promotes the release of inflammatory cytokines, including IL8 and TNF. Signals probably through G- proteins. Is a regulator of mast cell degranulation (PubMed:26841242).|
|Cellular Location||Cell membrane; Multi-pass membrane protein. Cell projection, ruffle membrane; Multi- pass membrane protein. Note=Localized at the leading edge of migrating cells.|
|Tissue Location||Expression is restricted to myeloid cells. Highest expression was found in peripheral blood leukocytes, followed by spleen and lymph nodes, with intermediate to low levels in thymus, bone marrow, fetal liver, placenta, and lung, and no expression in heart, brain, skeletal muscle, kidney, or pancreas. Expression is also detected in monocyte/macrophage and Jurkat cell lines but not in other cell lines tested. High expression in mast cells (PubMed:26841242)|
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Provided below are standard protocols that you may find useful for product applications.
EGF-like module-containing mucin-like receptor 2 (EMR2) is a member of the class B seven-span transmembrane (TM7) receptor family. EMR2 is expressed predominantly by cells of the immune system such as leukocytes. EMR2 is proteo-lytically cleaved into two subunits: a seven-transmembrane subunit, and an extracellular α subunit.
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