|Application ||WB, IHC|
|Reactivity||Human, Mouse, Rat, Bovine|
|Calculated MW||56795 Da|
|Application & Usage||Western blotting (0.5-4 µg/ml), Immunohistochemistry (5-20 µg/ml, frozen & paraffin sections, human thyroid). However, the optimal conditions should be determined individually. The antibody detects 57 kDa band in samples from human, mouse, rat, porcine, and ovine origins.|
|Other Names||EL, LIPG, EDL|
|Formulation||100 µg (0.5 mg/ml) affinity purified rabbit anti-Endothelial Lipase polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||Endothelial Lipase Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Has phospholipase and triglyceride lipase activities. Hydrolyzes high density lipoproteins (HDL) more efficiently than other lipoproteins. Binds heparin.|
|Tissue Location||High level of expression in the liver, placenta, lung, thyroid, kidney, testis and in the corpus luteum of the ovary. Expressed also in coronary artery endothelial cells, umbilical vein endothelial cells and in hepatocytes and osteosarcoma cell lines. Not detected in heart, brain and muscle|
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Provided below are standard protocols that you may find useful for product applications.
Endothelial lipase (EL) is a member of the triglyceride lipase gene family. It functions primarily as a phospholipase and has low triglyceride lipase activity. It was originally cloned from endothelial cells and found to be expressed in high level in the liver, placenta, lung, ovary, and macrophage. The wide spread distribution of this protein s µggests it plays a general role in lipid metabolism. Human endothelial lipase has an estimated molecular weight of 57 kDa.
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