|Calculated MW||41737 Da|
|Application & Usage||Western blotting (0.5-2 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes 45 kDa beta-Actin in samples from human and mouse origins. Reactivity to other species has not been tested.|
|Other Names||PS1TP5BP1 , ACTB, Beta-actin , b-actin|
|Formulation||100 µg (0.2mg/ml) protein A purified rabbit anti-β-Actin polyclonal antibody in phosphate-buffered saline (PBS) containing 0.5 % BSA, 30% glycerol, and 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||Beta-Actin Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.|
|Cellular Location||Cytoplasm, cytoskeleton. Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs|
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Provided below are standard protocols that you may find useful for product applications.
Actin is expressed in all eukaryotic cells and is the major component of the cytoskeleton. At least six types of actin are present in mammalian tissues and fall into three classes. Alpha actin expression is limited to various types of muscle and it regulates contactile potentials for the muscle cells, whereas beta and gamma actin, also known as cytoplasmic actin, are predominantly expressed in nonmuscle cells, controlling cell structure and motility.
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