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Beclin 1 Antibody

Rabbit Polyclonal Antibody

     
  • WB - Beclin 1 Antibody ABV10500-30
    Western blot analysis of Beclin 1 expression in Jurkat (Lane 1,2) and mouse intestine (lane 3) lysates.
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession O88597
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 51589 Da
Additional Information
Gene ID 56208
Application & Usage Western blotting (0.5-2 µg/ml). However, the optimal concentrations should be determined individually. Other applications have not been tested. The antibody recognizes ~53 kDa beclin 1 in Jurkat cell lysate. A weak band of ~110 kDa (possibly dimer) can also be observed in Jurkat cells. In mouse intestine tissue lysate, the antibody recognizes a ~100 kDa band (possibly dimer). Reactivity to other species has not been tested.
Other Names BECN1 , GT197 , Beclin-1 , beclin1 , ATG6 , VPS30
Target/Specificity Beclin 1
Antibody Form Liquid
Appearance Colorless liquid
Formulation 100 µg (0.2mg/ml) protein A purified rabbit anti-Beclin 1 polyclonal antibody in phosphate-buffered saline (PBS) containing 1% BSA, 50% glycerol, and 0.02% thimerosal.
Handling The antibody solution should be gently mixed before use.
Reconstitution & Storage -20 °C
Background Descriptions
PrecautionsBeclin 1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Becn1
Function Plays a central role in autophagy (PubMed:10604474, PubMed:12372286, PubMed:19270693). Acts as core subunit of different PI3K complex forms that mediate formation of phosphatidylinositol 3-phosphate and are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis (PubMed:19270693, PubMed:25275521). Involved in regulation of degradative endocytic trafficking and required for the abcission step in cytokinesis, probably in the context of PI3KC3-C2 (By similarity). Essential for the formation of PI3KC3-C2 but not PI3KC3-C1 PI3K complex forms (PubMed:25275521). Involved in endocytosis including endosome formation in neuronal cells (PubMed:25275521). May play a role in antiviral host defense (By similarity).
Cellular Location Cytoplasm. Golgi apparatus, trans-Golgi network membrane {ECO:0000250|UniProtKB:Q14457}; Peripheral membrane protein {ECO:0000250|UniProtKB:Q14457}. Endosome membrane {ECO:0000250|UniProtKB:Q14457}; Peripheral membrane protein {ECO:0000250|UniProtKB:Q14457}. Endoplasmic reticulum membrane {ECO:0000250|UniProtKB:Q14457}; Peripheral membrane protein {ECO:0000250|UniProtKB:Q14457}. Mitochondrion membrane {ECO:0000250|UniProtKB:Q14457}; Peripheral membrane protein {ECO:0000250|UniProtKB:Q14457}. Endosome. Cytoplasmic vesicle, autophagosome. Note=Interaction with ATG14 promotes translocation to autophagosomes (By similarity). Expressed in dendrites and cell bodies of cerebellar Purkinje cells. Localized to endosomes in neurons (PubMed:25275521). {ECO:0000250|UniProtKB:Q14457, ECO:0000269|PubMed:12372286, ECO:0000269|PubMed:25275521} Beclin-1-C 37 kDa: Mitochondrion
Research Areas
Citations (0)

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Background

Beclin is a Bcl-2 binding protein. Its structure includes a Bcl-2 binding coiled-coil region, and a leucine-rich nuclear export signal (NES). Beclin protein colocalizes with intracytoplasmic organelles and nuclei in normal COS7 and MCF7 cells. Beclin interaction with Bcl-2 may be involved with host viral defense, since over expression of beclin inhibits Sindbis virus replication and expression of beclin lacking the Bcl-2 binding domain has no antiviral effects.

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$ 89.00
$ 245.00
Cat# ABV10500-30
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