|Reactivity||Human, Mouse, Rat|
|Calculated MW||62308 Da|
|Application & Usage||Western blotting (0.5-4 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes ~80-85 kDa doublet of TME8 in samples from human, mouse and rat origins. Reactivity to other species has not been tested.|
|Other Names||Tumor Endothelial Marker 8 , TEM-8, TEM8 , ATR , FLJ10601 , FLJ11298 , FLJ21776|
|Formulation||100 µg (0.2mg/ml) affinity purified rabbit anti-TEM8/ATR1 polyclonal antibody in phosphate-buffered saline (PBS) containing 0.5% BSA, 30% glycerol, and 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||TEM-8/ATR1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Plays a role in cell attachment and migration. Interacts with extracellular matrix proteins and with the actin cytoskeleton. Mediates adhesion of cells to type 1 collagen and gelatin, reorganization of the actin cytoskeleton and promotes cell spreading. Plays a role in the angiogenic response of cultured umbilical vein endothelial cells (By similarity).|
|Cellular Location||Cell membrane; Single-pass type I membrane protein. Cell projection, lamellipodium membrane; Single-pass type I membrane protein. Cell projection, filopodium membrane; Single-pass type I membrane protein Note=At the membrane of lamellipodia and at the tip of actin- enriched filopodia. Colocalizes with actin at the base of lamellipodia (By similarity).|
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Tumor Endothelial Markers (TEMs, TEM 1-9) are recently identified genes, whose expression is elevated in tumor-associated endothelium. TEMs are barely detectable in normal endothelial cells. TEM8 has been shown to interact with C5 domain of collagen a3(VI), a protein also preferentially expressed in tumor endothelium. It is tho µght that TEM8 may play a role in tumor angiogenesis.
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